Factors and mechanisms of mobility of the high pathogenicity island of Yersinia [Elektronische Ressource] / von Uladzimir Antonenka

Max von Pettenkofer-Institut für Hygiene und M edizinische M ikrobiologieLudwig -Maximilians-Universität MünchenDirektor: Prof. Dr. Dr. J. HeesemannFactors and Mechani sms of Mobi lity of the H igh P athogenicity Island of Y ersinia Dissertation zurErlangung d es Doktorgrades derFakultät für Biologie derLudwig-Maximilians-Universität MünchenvonUladzimir Antonenkaaus Gomel, BelarusMünchen 2007Dissertation e ingereicht am: 13. Juni 2007Vorsitzender: Prof. D r. E lizabeth W eissGutachter: Prof. D r. A nton H artmannGutachter: Prof. D r. H einrich JungProtokoll: Prof. D r. M akoto H ayashiSondervotum: Prof. D r. D r. Jürgen H eesemannTag de r mündlichen Prüfung: 17. D ezember 2007TABLE OF CONTENTS IA. INTRODUCTION 11. General characteristics of Yersinia species 12. The concept of “Pathogenicity Island” (PAI) 23. PAIs, as a particular case of “Genomic Islands” (GEIs) 34. Evolution of genomic islands 45. GEIs strategies for lateral transfer 66. Structure and function of the HPI 76.1 Yersiniabactin core 86.2 Mobility of the Yersinia HPI 107. Aims of this research study 13B. MATERIALS AND METHODS 141. Material 141.1 Equipment 141.2 Other materials 151.3 Chemicals and Enzymes 152. Bacteria, Plasmids and Primers 152.1 Bacterial strains and plasmids 152.2 List of primers 173. Culture media, Antibiotics, Strain Cultivation and Storage 193.1 Culture media 193.2 Antibiotics 213.3 Cultivation and long term storage of bacteria 214.