Target site pharmacokinetics of antiinfectives in the treatment of serious gram positive infections [Elektronische Ressource] / von Nele Plock

Target Site Pharmacokinetics of Antiinfectives in the Treatment of Serious Gram-positive Infections Dissertation zur Erlangung des akademischen Grades doctor rerum naturalium (Dr.rer.nat.) Vorgelegt der Naturwissenschaftlichen Fakultät I / Biowissenschaften der Martin-Luther-Universität Halle-Wittenberg von Nele Plock geb. am 17.08.1978 in Oldenburg (Oldb) Gutachter: 1. Prof. Dr. Charlotte Kloft 2. Prof. Dr. Hans-Hubert Borchert 3. Prof. Dr. Andreas Langner Halle (Saale), 22. Februar 2007 urn:nbn:de:gbv:3-000011463[http://nbn-resolving.de/urn/resolver.pl?urn=nbn%3Ade%3Agbv%3A3-000011463] Für Paps. iii Abstract This thesis aimed at investigating the pharmacokinetics (PK) of antiinfectives at the site of infection. This objective was pursued in three different projects. In the first project, vancomycin andlinezolid were studied in in vitro experiments to enable the conduction of clinical trials investigating their PK by using the microdialysis approach. A rapid and reliable HPLC assay capable of measuring vancomycin concentrations in microdialysate and plasma was developed. Moreover, a previously existing analytical assay for linezolid was extended to the matrices urine, bone marrow, bone biopsy samples and bone microdialysate.