Microtubule associated proteins in fission yeast [Elektronische Ressource] / vorgelegt von Lindsay J. Murrells

INAUGURAL - DISSERTATION zur Erlangung der Doktorwürde der Naturwissenschaftlich-Mathematischen Gesamtfakultät der Ruprecht-Karls-Universität Heidelberg vorgelegt von Lindsay J. Murrells Tag der mündlichen Prüfung: 16. Oktober 2008 DISSERTATION submitted to the Combined Faculties for the Natural Sciences and for Mathematics of the Ruperto-Carola University of Heidelberg, Germany for the degree of Doctor of Natural Sciences presented by Lindsay J. Murrells thDate of oral examination: 16 October 2008 Microtubule-associated proteins in fission yeast Referees: Dr. Darren Gilmour Prof. Elmar Schiebel Summary The highly conserved Dis1/XMAP215 family of microtubule-associated proteins (MAPs) play a central role in cytoplasmic microtubule organisation and mitotic spindle formation. The fission yeast S. pombe has two family members, Alp14 and Dis1. Both localise to interphase microtubules, spindle pole bodies (the yeast equivalent of the centrosome), and kinetochores. Here we present the characterisation of Alp14 and Dis1 during interphase. We find that Alp14 localisation resembles that of Mal3, a canonical plus end tracking protein. Deletion results in a decrease in the number and length of interphase microtubule bundles at low temperatures. Alp14 is temperature sensitive.