Isolation and characterisation of the intermembrane space components of the mitochondrial TIM22 protein import machinery of Neurospora crassa [Elektronische Ressource] / von Andreja Vasiljev

Isolation and characterisation of the intermembrane space components of the mitochondrial TIM22 protein import machinery of Neurospora crassa Dissertation zur Erlangung des doktorgrades der Fakultät für Biologie der Ludwig-Maximilians-Universität München von Andreja Vasiljev aus Subotica, Serbien und Montenegro München, 2004 Mündliche Prüfung am: 15.11.2004Sondergutachter: Herr Prof. Dr.Dr. Walter Neupert1. Gutachter: Herr Prof. Dr. Reinhold Herrmann2. Gutachter: Herr PD Dr. Enrico Schleiff To my grandmothers, Jelena and Marija Table of Contents 1. Introduction ....................................................................................1 1.1. Mitochondrial protein translocation machineries........................................................ 1 1.1.1. Mitochondrial structure and function................................................................. 1 1.1.2. Protein translocation in mitochondria of N. crassa and S. cerevisiae................ 4 1.2.2.1. Targeting of preproteins to mitochondria.......................................................... 4 1.2.2.2. Translocases of the outer mitochondrial membrane ......................................... 6 1.2.2.3. Translocases of the inner me 8 1.2. Zinc fingers........................................