Growth zone instability in T-shaped Schizosaccharomyces pombe cells [Elektronische Ressource] / presented by Ioannis Legouras

Dissertationsubmitted to theCombined Faculties for the Natural Sciences and for Mathematicsof the Ruperto-Carola University of Heidelberg, Germanyfor the degree ofDoctor of Natural Sciencespresented byDiplom-Biologe Ioannis Legourasborn in: Patra, GreeceOral-examination: ..............................Growth zone instability in T-shapedSchizosaccharomyces pombe cellsSupervisor:Dr. Fran cois NedelecReferees:Dr. Damian BrunnerDr. Jochen WittbrodtContents1 Introduction 51.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51.2 The Cytoskeleton . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61.3 An introduction to Schizosaccharomyces pombe . . . . . . . . . . . . . . . 71.3.1 History and General information . . . . . . . . . . . . . . . . . . . 71.3.2 Establishment of polarity in S.pombe . . . . . . . . . . . . . . . . . 71.4 Microtubule-dependent growth in S.pombe . . . . . . . . . . . . . . . . . . 91.4.1 MT plus-end tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101.4.2 Polarity machinery . . . . . . . . . . . . . . . . . . . . . . . . . . . 131.4.3 Ectopic Growth Studies . . . . . . . . . . . . . . . . . . . . . . . . 261.5 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 281.6 Methods for shape change . . . . . . . . . . . . . . . . . . . . . . . . . . . 291.7 Aim of Thesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .