Characterization of Staphylococcus aureus peptidoglycan hydrolases and isolation of defined peptidoglycan structures [Elektronische Ressource] / von Raja Biswas

Characterization of Staphylococcus aureus peptidogylcan hydrolases and isolation of defined peptidoglycan structures der Fakultät für Biologie der Eberhard Karls Universität Tübingen zur Erlangung des Grades eines Doktors der Naturwissenschaften von Raja Biswas aus Bishnupur, Indien vorgelegte Dissertation 2006 Tag der mündlichen Prüfung: 27.07.2006 Dekan: Prof. Dr. F. Schöffl 1. Berichterstatter: Prof. Dr. F. Götz 2. Berichterstatter: Prof. Dr. V. Braun … for my parents INDEX INDEX A. SUMMARY 1 B. INTRODUCTION 3 C. MATERIAL AND METHODS 12 1. Materials 12 1.1 Chemicals, kits and instruments 12 1.1.1 Chemicals and kits 12 1.1.2 Instruments 13 1.2 Software 14 1.2.1 Purchased software 14 1.2.2 Sequence analysis websites 14 1.3 Nucleotide sequence accession numbers 14 1.4 Antibiotics 14 1.5 Strains and plasmids 15 1.5.1 Strains 15 1.5.2 Plasmids 15 1.6 Oligo-neucleotides 16 1.6.1 Synthetic oligo-neucleotides for PCR 16 1.6.2 Synthetic oligonucleotides for DNA sequencing (5’ labeled) 16 1.7 Media 17 1.8 Buffers of Molecular Cloning 17 1.8.1 Plasmid extraction buffers 17 1.8.2 Solutions for protoplast transformation 18 1.8.3 Agarose gel electrophoresis buffers 19 1.8.4 Buffers for E.